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- Title
New insights into the activation of o-xylene biodegradation in Pseudomonas stutzeri OX1 by pathway substrates.
- Authors
Arenghi, Fabio L.G.; Barbieri, Paola; Bertoni, Giovanni; de Lorenzo, Victor
- Abstract
The regulation of the tou operon of Pseudomonas stutzeri OX1, for degradation of toluene and o-xylene via phenolic intermediates, has been faithfully reconstructed in vitro with purified proteins. The set-up included the prokaryotic enhancer-binding protein TouR, the σ54-dependent PToMO promoter and the σ54-containing RNA polymerase. With this system we prove that direct binding of 2-methylphenol (o-cresol) to Tour is the only regulatory step for activation of PToMo in response to aromatic effectors, thereby ruling out the involvement of other factors or a need for protein processing. In addition, we found that while Tour failed entirely to activate ProMo in the absence of inducers, the protein had per se a very significant ATPase activity, which was only moderately increased by o-cresol addition. The results presented here support the view that TouR-like proteins are particularly suitable as evolutionary assets to endow recently evolved pathways for the degradation of environmental pollutants with an optimal degree of transcriptional regulation.
- Subjects
PSEUDOMONAS; TOLUENE; XYLENE; PROTEINS; RNA; TRANSFERASES; POLLUTANTS
- Publication
EMBO Reports, 2001, Vol 2, Issue 5, p409
- ISSN
1469-221X
- Publication type
Article
- DOI
10.1093/embo-reports/kve092