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- Title
Salts Induced Salinity and in Vitro Multiplication of Paronychia argentea.
- Authors
Osman, Nahid Abd Elhamid; Shatnawi, Mohamad; Shibli, Rida; Majdalawi, Majdi; Al Tawaha, Abdel Rahman M.; Qudah, Tamara
- Abstract
Paronychia argentea is a wild herb plant with a high medicinal value. P. argentea plant is a neglected herb that grows without any attention in terms of research for cultivation and propagation. The conventional propagation methods of P. argentea by seeds and cutting are not preferred due to low germination percentage and cutting rooting problems. As a substitute for seed propagation, effective micropropagation protocols were developed. Using 0.6 mg/L 6-Benzylaminopurine (BAP), maximum of 3.90 (shoot/explant) was produced on Murashige and Skoog (MS) medium. The in vitro growth was significantly decreased with increasing NaCl concentration. The potassium (K) and nitrogen (N) concentrations in P. argentea plantlets decreased significantly under NaCl treatment. The level of protein in leaf tissue generally decreased with increased NaCl concentrations in the medium. The proline content in P. argentea plantlets increased significantly along with NaCl concentrations. An increase in NaCl concentration in the medium resulted in an increase in total soluble solids (TSS) in plant tissue. Moreover, as salinity level concentration increased, relative water content was decreased. High NaCl was significantly affected the in vitro growth of plants. It was shown that in vitro P. argentea plantlets could be tolerant to in vitro salinity.
- Subjects
PARONYCHIA; PLANT micropropagation; BENZYLAMINOPURINE; GERMINATION; SALINITY
- Publication
Ecological Engineering & Environmental Technology (EEET), 2021, Vol 22, Issue 5, p55
- ISSN
2719-7050
- Publication type
Article
- DOI
10.12912/27197050/139408