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- Title
STUDIES OF SELECTIVE TISSUES DEVELOPMENT OF PROTEIN TYROSINE PHOSPHATASES AND EFFECT OF OXIDATIVE STRESS IN ARACHIS HYPOGAEA L.
- Authors
kumar, Pradeep; Saini, Sangeeta; Bansal, Divya; Chand, Mukesh; Sharma, Hari Om
- Abstract
Phosphorylation of tyrosine residues in protein has been envisaged to play a crucial role in the regulation of a variety of cellular processes, including the signal transduction and development in higher plants. The protein phosphorylation is around regulated by both protein kinases and specific protein phosphatases. The activity of Kinases and phosphatases can be modulated by the oxidation or reduction. The oxidative stress-mediated oxidation of PTP by hydrogen peroxide, superoxide or by hydroxyl radical could regulate the transduction of signals from the membrane to the nucleus via the modulation of cellular enzymatic activity. The present studies carried out that the peanut (Arachis hypogaea L.) seedling grown in dark on moistened whatman filter paper at 28±2°C, localized tissue specific multi-cellular forms of protein tyrosine phosphatases (PTPases). The level of PTPases was increased by several-fold during the 6 to 10 days of germination period. The oxidative stress is carried out by treating seedlings with 1% H2O2 for 2-3 hours, resulted in 2.0 fold increases in the level of PTPase activity, which was followed by slowly decline in the PTPase activity and 4 hours treatment, the level of PTPase activity was decreased as compared to the untreated seedlings. The specific activity (unit/mg-protein) of PTPase was increased in the order Cotyledon, Epicotyls, Roots and highest in Hypocotyls when different part of seedlings were treated with 1% H2O2 for 3 hours. The specific activity was decreased as compared to untreated seedling. These results suggest that the most of signaling pathways may be influenced by oxidative stress of protein tyrosine phosphatases, consequently leading to an aberrant phosphorylation state of cellular proteins and role of protein tyrosine phosphatases in stress-related cellular processes.
- Subjects
PEANUTS; PHOSPHATASES; OXIDATIVE stress; PHOSPHORYLATION; CELLULAR signal transduction
- Publication
Plant Archives (09725210), 2021, Vol 21, Issue 1, p1694
- ISSN
0972-5210
- Publication type
Article
- DOI
10.51470/PLANTARCHIVES.2021.v21.no1.232