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- Title
Expression of Macrophage Scavenger Receptor (MSR1) in Peripheral Blood Cells from Patients with Different Respiratory Diseases: Beyond Monocytes.
- Authors
Baos, Selene; Cremades-Jimeno, Lucía; López-Ramos, María; de Pedro, María Ángeles; Uriarte, Silvia A.; Sastre, Joaquín; González-Mangado, Nicolás; Rodríguez-Nieto, María Jesús; Peces-Barba, Germán; Cárdaba, Blanca
- Abstract
Background: Macrophage scavenger receptor 1 (MSR1) has mostly been described in macrophages, but we previously found a significant gene expression increase in peripheral blood mononuclear cells (PBMCs) of asthmatic patients. Objective: To confirm those results and to define its cellular origin in PBMCs. Methods: Four groups of subjects were studied: healthy controls (C), nonallergic asthmatic (NA), allergic asthmatic (AA), and chronic obstructive pulmonary disease (COPD) patients. RNA was extracted from PBMCs. MSR1 gene expression was analyzed by RT-qPCR. The presence of MSR1 on the cellular surface of PBMC cellular subtypes was analyzed by confocal microscopy and flow cytometry. Results: MSR1 gene expression was significantly increased in the three clinical conditions compared to the healthy control group, with substantial variations according to disease type and severity. MSR1 expression on T cells (CD4+ and CD8+), B cells, and monocytes was confirmed by confocal microscopy and flow cytometry. In all clinical groups, the four immune cell subtypes studied expressed MSR1, with a greater expression on B lymphocytes and monocytes, exhibiting differences according to disease and severity. Conclusions: This is the first description of MSR1's presence on lymphocytes' surfaces and reinforces the potential role of MSR1 as a player in asthma and COPD.
- Subjects
BLOOD cells; MONONUCLEAR leukocytes; RESPIRATORY diseases; MONOCYTES; CHRONIC obstructive pulmonary disease
- Publication
Journal of Clinical Medicine, 2022, Vol 11, Issue 5, p1439
- ISSN
2077-0383
- Publication type
Article
- DOI
10.3390/jcm11051439