We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Monocationic surfactant induced ultra structural changes in antibiotic resistant Escherichia coli.
- Authors
Deo, A. A.; Kulkarni, A. S.; Meshram, S. U.
- Abstract
Background & objectives: Cetrimide is a monoeationic surfactant, commonly used for disinfection of hospital floors, equipments, for cleansing of burns and wounds, hand wash, etc. We evaluated whether antibiotic resistant (AR) Escherichia coli isolates from hospital settings (nosocomial pathogens) show any evidence of significant reduction in their susceptibility to cetrimide. Also the response of AR E. coli (nosoeomial pathogens) to the action of cetrimide was assessed by studying the ultra structural changes induced using transmission electron microscopy (TEM). Methods: A total of 165 clinical samples were screened for isolation of E. coli. Eighty two (49.6%) samples were positive for E. coli Antibiotic susceptibility testing of E. coli isolates was carried out by Kirby Bauer method to isolate AR E. coli. The randomly selected AR E. coli isolate was treated with different concentrations of cetrimide and minimum inhibitory concentration (MIC) of cetrimide was determined by broth micro dilution method. This same isolate was used for performing time kill assay and TEM study. Results: The test E. coli isolate showed resistance to 12 different antibiotics. The MIC of cetrimide against ARE. coli was 312.5 µg/ml. The ultra cellular structural changes in cetrimide treated AR E. coli revealed vacuole formation, disaxilization of nuclear material, loss of cytoplasmic granularity, bleb formation and cell lysis. Conclusion: Ultra structural changes induced by the action of cetrimide revealed cell damaging changes in the AR E. coli to be dose and time dependent. The results showed that antibiotic resistance does not alter any change in susceptibility of E. coli to cetrimide, which was found to be still an effective disinfectant against a nosocomial pathogen E. coli.
- Subjects
PHYSIOLOGICAL effects of surface active agents; ESCHERICHIA coli physiology; DRUG resistance in microorganisms; MICROORGANISMS -- Ultrastructure; PATHOGENIC microorganisms; TRANSMISSION electron microscopy
- Publication
Indian Journal of Medical Research, 2010, Vol 131, Issue 6, p825
- ISSN
0971-5916
- Publication type
Article