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- Title
Evaluation of α-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia.
- Authors
Juri Kim; Myeong Heon Shin; Kyoung-Ju Song; Soon-Jung Park
- Abstract
The α/β-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant &alpha-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of e-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the α-tubulin gene from G. lamblia. PCR-RFLP analysis of this α-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that α-tubulin can be used as a molecular probe to detect G. lamblia.
- Subjects
GIARDIA lamblia; MICROTUBULES; TUBULINS; IMMUNOFLUORESCENCE; IMMUNOGLOBULINS; POLYMERASE chain reaction; RESTRICTION fragment length polymorphisms
- Publication
Korean Journal of Parasitology, 2009, Vol 47, Issue 3, p287
- ISSN
0023-4001
- Publication type
Article
- DOI
10.3347/kjp.2009.47.3.287