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- Title
Cardiac pathophysiology: real-time PCR detection of cryptosporidium parvum infection in camels.
- Authors
sabbar, Khawla hussien; Kareem, Safaa M.; Abbas, Fatima Hashim
- Abstract
Cryptosporidium's significance in the realm of public health and economics lies in its capacity to induce cryptosporidiosis and resultant financial losses stemming from animal diseases. The primary objective of this investigation was to ascertain the prevalence of Cryptosporidium infection in camels inhabiting the arid regions of western Iraq, and to understand its cardiac physiology that enables it to thrive in such extremely dry conditions. This was accomplished through the isolation and diagnosis of Cryptosporidium parvum utilizing the real-time polymerase chain reaction (PCR) technique. The study, conducted in the Qadisiyah province, spanned a duration of 6 months, from November 2022 to April 2022. A total of 50 fecal samples were collected from camels, evenly distributed among both sexes, and categorized into three age groups: less than one year, 1-2 years, and 2-10 years. DNA extraction was performed using a stool DNA extraction Kit, and the obtained DNA samples underwent Real-Time PCR analysis, relying on specific primers designed for the heat shock protein gene in Cryptosporidium parvum. These primers were developed based on data from the NCBI-Genbank database (Genbank code: GQ259151.1) and primer3 plus. The findings revealed a Cryptosporidium parvum infection rate of 24%, with 12 out of 50 samples testing positive, and a remarkable Real-Time PCR efficiency of 99%. This infection was observed in both male (16.66%) and female (30.76%) camels, with a higher infection rate detected in adult camels (47.05%), followed by camels aged less than one year (14.28%), and a 10.52% infection rate in the young stage. Significant differences (p<0.05) were observed in relation to age and gender.
- Subjects
IRAQ; CRYPTOSPORIDIUM parvum; CRYPTOSPORIDIOSIS; CAMELS; HEAT shock proteins; ANIMAL diseases
- Publication
Revista Latinoamericana de Hipertension, 2023, Vol 18, Issue 10, p516
- ISSN
1856-4550
- Publication type
Article
- DOI
10.5281/zenodo.10443671