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- Title
Lactate dehydrogenase (LDH) isoenzymes and proliferative activity of lymphoid cells -- an immunocytochemical study.
- Authors
Pan, L.; Beverley, P. C. L.; Isaacson, P. G.
- Abstract
In a recent immunohistochemical study, we suggested that elevation of LDH isoenzymes is generally related to cell proliferation. To explore this relationship further, we have now examined the expression of H- and M-type LDH isoenzymes immunocytochemically in human resting and mitogen-activated B and T lymphocytes. In the resting state. T lymphocytes showed strong staining for H-type LDH but showed little or no staining for M-type LDH. while B lymphocytes showed only weak staining for M-type LDH, During activation of T cells, M-type LDH started to increase when cells entered the early stages of the cell cycle. The staining intensity increased to a maximum when the percentage of the T cells at the S/G2/M phases of the cell cycle reached its peak, M-type LDH expression declined when the activated T cells returned to their resting state. Staining for H-type LDH remained strong in T cells during activation. In B lymphocytes, both H- and M-type LDH isoenzymes increased concomitantly following activation and the staining intensity also correlated well with the percentage of the S/G2 M fraction. The expression of H- and M-type LDH was also determined in fresh leukaemia and a variety of lymphoid ceil lines. It was noted that cells of chronic lymphocytic leukaemia (CLL) and pro-lymphocytic leukaemia (PLL), morphologically similar to normal lymphocytes, showed a LDH staining pattern resembling that of resting B lymphocytes, while lymphoblasts in T cell acute lymphoblastic leukaemia (T-ALL). high grade B cell lymphoma and Epstein Barr virus (EBV) transformed cell lines showed a LDH staining pattern similar to that in activated T or B lymphocytes. Taken together, our results have demonstrated a significant correlation between expression of LDH and proliferative activity of cells. Immunostaining with the MoAbs to H- and M-type LDH can, therefore, provide a useful means not only for identification of T and B lymphocytes but also for rapid evaluation of the proliferating fraction of normal and neoplastic human cell populations.
- Subjects
LACTATE dehydrogenase; ISOENZYMES; IMMUNOHISTOCHEMISTRY; B cells; GENE expression; LYMPHOCYTES
- Publication
Clinical & Experimental Immunology, 1991, Vol 86, Issue 2, p240
- ISSN
0009-9104
- Publication type
Article