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- Title
Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication Nagao Hayashi et al. Durable panicle blast resistance gene Pb1.
- Authors
Hayashi, Nagao; Inoue, Haruhiko; Kato, Takahiro; Funao, Taketo; Shirota, Masaki; Shimizu, Takehiko; Kanamori, Hiroyuki; Yamane, Hiroko; Hayano-Saito, Yuriko; Matsumoto, Takashi; Yano, Masahiro; Takatsuji, Hiroshi
- Abstract
Rice blast is one of the most widespread and destructive plant diseases worldwide. Breeders have used disease resistance ( R) genes that mediate fungal race-specific 'gene-for-gene' resistance to manage rice blast, but the resistance is prone to breakdown due to high pathogenic variability of blast fungus. Panicle blast 1 ( Pb1) is a blast-resistance gene derived from the indica cultivar 'Modan'. Pb1-mediated resistance, which is characterized by durability of resistance and adult/panicle blast resistance, has been introduced into elite varieties for commercial cultivation. We isolated the Pb1 gene by map-based cloning. It encoded a coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein. The Pb1 protein sequence differed from previously reported R-proteins, particularly in the NBS domain, in which the P-loop was apparently absent and some other motifs were degenerated. Pb1 was located within one of tandemly repeated 60-kb units, which presumably arose through local genome duplication. Pb1 transcript levels increased during the development of Pb1+ cultivars; this expression pattern accounts for their adult/panicle resistance. Promoter:GUS analysis indicated that genome duplication played a crucial role in the generation of Pb1 by placing a promoter sequence upstream of its coding sequence, thereby conferring a Pb1-characteristic expression pattern to a transcriptionally inactive 'sleeping' resistance gene. We discuss possible determinants for the durability of Pb1-mediated blast resistance.
- Subjects
AGRICULTURAL pests; AMINO acids; GENETICS; PROTEINS; GENOMES
- Publication
Plant Journal, 2010, Vol 64, Issue 3, p498
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2010.04348.x