We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
White-core endospermfloury endosperm-4in rice is generated by knockout mutations in the C<sub>4</sub>-type pyruvate orthophosphate dikinase gene (OsPPDKB).
- Authors
Hong-Gyu Kang; Sunhee Park; Makoto Matsuoka; Gynheung An
- Abstract
We have isolated afloury endosperm-4(flo4) rice mutant with a floury-white endosperm but a normal outer portion. Scanning electron microscopic analysis revealed that this abnormal endosperm consisted of loosely packed starch granules. The mutant phenotype was generated by T-DNA insertion into the fifth intron of theOsPPDKBgene encoding pyruvate orthophosphate dikinase (PPDK). Plants containingflo4-1produced noOsPPDKBtranscript or the OsPPDKB protein in their developing kernels and leaves. We obtained two additional alleles,flo4-2andflo4-3, that also showed the same white-core endosperm phenotype. Theflo4kernels weighed about 6% less than wild-type ones. Starch contents in both kernel types were similar, but the total protein content was slightly higher in the mutant kernels. Moreover, lipid contents were significantly increased in theflo4kernels. Expression analyses demonstrated that the cytosolic mRNA ofOsPPDKBwas induced in the reproductive organs after pollination, and greatly increased until about 10 days after fertilization. This mRNA was localized mainly in the endosperm, aleurone, and scutellum of the developing kernel. Our results suggest that cytosolic PPDK functions in rice to modulate carbon metabolism during grain filling.
- Subjects
RICE genetics; STARCH; PYRUVATES; PLANT self-incompatibility; PLANT genetics
- Publication
Plant Journal, 2005, Vol 42, Issue 6, p901
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2005.02423.x