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- Title
Aspirin-induced gastrointestinal damage is associated with an inhibition of epithelial cell autophagy.
- Authors
Hernández, Carlos; Barrachina, Maria; Vallecillo-Hernández, Jorge; Álvarez, Ángeles; Ortiz-Masiá, Dolores; Cosín-Roger, Jesús; Esplugues, Juan; Calatayud, Sara; Hernández, Carlos; Barrachina, Maria Dolores; Vallecillo-Hernández, Jorge; Álvarez, Ángeles; Ortiz-Masiá, Dolores; Cosín-Roger, Jesús; Esplugues, Juan Vicente
- Abstract
<bold>Background: </bold>Aspirin (ASA) causes gastrotoxicity by hampering the epithelial defense against luminal contents through cyclooxygenase inhibition. Since cell survival in tough conditions may depend on rescue mechanisms like autophagy, we analyzed whether epithelial cells rely on this process to defend themselves from aspirin's damaging action.<bold>Methods: </bold>Rats received a single dose of ASA (150 mg/kg, p.o.) with or without pretreatment with the autophagy inhibitor 3-methyladenine, and gastric injury and epithelial autophagy were evaluated 3 h later. The effects of ASA on cell viability and autophagy were also evaluated in gastric epithelial AGS cells.<bold>Results: </bold>Basal autophagy in the gastric mucosa was inhibited by ASA as demonstrated by increased levels of p62 and ubiquitinated proteins and total LC3 and a reduced LC3-II/LC3-I ratio. Similarly, ASA increased p62 and decreased LC3-II accumulation and the number of EmGFP/LC3B puncta in AGS cells. ASA activated the PI3K/Akt-GSK3-mTOR pathway, which phosphorylates ULK1 to prevent autophagy initiation, changes that were inhibited by the PI3K-inhibitor wortmannin. Autophagy inhibition seems to enhance the vulnerability of gastric epithelial cells as a combination of ASA with 3-methyladenine exacerbated rat gastric damage and AGS cell apoptosis.<bold>Conclusions: </bold>Our data highlight the importance of autophagy in the gastric mucosa as a protective mechanism when the epithelium is injured. In the stomach, aspirin induces mucosal damage and reduces autophagy, thus, eliminating a protective mechanism that epithelial cells could use to escape death. We hypothesize that the combination of aspirin with drugs that activate autophagy could protect against gastric damage.
- Subjects
GASTROINTESTINAL diseases; EPITHELIAL cells; AUTOPHAGY; ASPIRIN; CYCLOOXYGENASES; UBIQUITINATION
- Publication
Journal of Gastroenterology, 2016, Vol 51, Issue 7, p691
- ISSN
0944-1174
- Publication type
journal article
- DOI
10.1007/s00535-015-1137-1