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- Title
DAPT suppresses the proliferation of human glioma cell line SHG-44.
- Authors
Liu, Xin; Xu, Qiu-Ran; Xie, Wan-Fu; Wang, Mao-De
- Abstract
Objective To explore the suppressing effect of γ-secretase inhibitor DAPT on proliferation of human glioma cell line SHG-44 in vitro and its mechanism.Methods The SHG-44 cell was treated by DAPT with different concentration. The proliferation of cells was detected by MTT assay; cell cycle and TSC of CD133+ were determined by flow cytometry analysis technique; the key factor in Notch signaling pathway (Notch-1, Delta-1, Hes-1) was measured by reverse transcrip tase-polymerase chain reaction and western blotting.Results DAPT inhibited the growth and proliferation of SHG-44 cells significantly(P<0.05). And the inhibiting effect on SHG-44 cells produced by DAPT showed a dose-dependent manner. DAPT increased the rate of cells in G0/G1 phase of SHG-44 cells, while it decreased the rate of cells in S phase. TSC of CD133+ was significantly reduced after DAPT treated SHG-44 cells. The expression of protein and mRNA of Notch-1, Delta-1 and Hes-1 were gradually downregulated with the increase of DAPT doses.Conclusions DAPT can downregulate these key factor in Notch signaling pathway, reduce the TSC of CD133+ and inhibit the proliferation of SHG-44 cells.
- Publication
Asian Pacific Journal of Tropical Medicine, 2014, Vol 7, Issue 7, p552
- ISSN
1995-7645
- Publication type
Article
- DOI
10.1016/S1995-7645(14)60092-4