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- Title
Effects of epigallocatechin‑3‑gallate on iron metabolism in spinal cord motor neurons.
- Authors
FENGYUAN CHE; GUANGYING WANG; JIXU YU; XIANJUN WANG; YUCHENG LU; QINGXI FU; QUANPING SU; JIANZHANG JIANG; YIFENG DU
- Abstract
Accumulating evidence suggests that iron homeostasis is disordered in amyotrophic lateral sclerosis (ALS). In view of the promising performance of epigallocatechin‑3‑gallate (EGCG) in neuroprotection studies, the present study aimed to verify whether EGCG protects motor neurons in an ALS model, and whether it has any effects on iron metabolism using an ELISA and western blotting. The results demonstrated that EGCG decreased oxidative stress and protected motor neurons in the organotypic culture of the rat spinal cord. Furthermore, total iron levels increased significantly in the spinal cord following 3 weeks of treatment with threo‑hydroxyaspartate. In addition, the expression of influx proteins (transferrin receptor and divalent metal‑ion transporter 1) increased significantly. However, EGCG demonstrated no effect on total iron levels and the expression of influx proteins. In conclusion, EGCG leads to a decrease in oxidative stress levels, leading to motor neuron protection in the organotypic culture of a rat spinal cord; however, EGCG does not alter iron metabolism protein expression regulation.
- Subjects
EPIGALLOCATECHIN gallate; GENETICS of amyotrophic lateral sclerosis; AMYOTROPHIC lateral sclerosis; OXIDATIVE stress; MOTOR neurons; PATIENTS
- Publication
Molecular Medicine Reports, 2017, Vol 16, Issue 3, p3010
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2017.6919