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- Title
Effect of interferon-γ, interleukin-10, lipopolysaccharide and tumor necrosis factor-α on chitotriosidase synthesis in human macrophages.
- Authors
Di Rosa, Michelino; Musumeci, Maria; Scuto, Anna; Musumeci, Salvatore; Malaguarnera, Lucia
- Abstract
Human chitotriosidase is a chitinolytic enzyme and mainly produced by activated macrophages. Recently, we observed that prolactin, which is structurally related to several cytokines and is involved in regulating monocyte/macrophage functions, upregulates chitotriosidase gene expression in human macrophages, suggesting that chitotriosidase is not only a biochemical marker of macrophage activation in lysosomal diseases and hematological disorders, but also may reflect induction of an immunological response. To confirm this hypothesis we evaluated by quantitative real-time PCR the mRNA chitotriosidase levels in human monocytes/macrophages following treatment with pro-inflammatory stimuli such as interferon-γ, tumor necrosis factor-α, lipopolysaccharide, and interleukin-10, an anti-inflammatory cytokine. Stimulation of macrophages with interferon-γ, tumor necrosis factor-α and lipopolysaccharide resulted in increased levels of chitotriosidase mRNA, as well as chitotriosidase activity, whereas interleukin-10 decreased chitotriosidase synthesis. This finding is consistent with the hypothesis that the production of chitotriosidase by macrophages could have biological relevance in the immune response.
- Subjects
MACROPHAGES; INTERFERONS; CYTOKINES; MESSENGER RNA; TUMOR necrosis factors; IMMUNE response
- Publication
Clinical Chemistry & Laboratory Medicine, 2005, Vol 43, Issue 5, p499
- ISSN
1434-6621
- Publication type
Article
- DOI
10.1515/CCLM.2005.088