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- Title
Expression of p27/kip1 is down-regulated in human prostate carcinoma progression.
- Authors
Fernández, Pedro L.; Arce, Yolanda; Farré, Xavier; Martínez, Antonio; Nadal, Alfons; Rey, María J.; Peiró, Nerea; Campo, Elías; Cardesa, Antonio
- Abstract
p27Kip1 is a cyclin-dependent kinase inhibitor whose down-regulation has been observed in several tumour models, including breast, colorectal, and gastric carcinomas. The purpose of this study was to assess p27Kip1 protein expression in normal and benign prostatic epithelia as well as the possible existence of abnormalities in prostate carcinoma progression. p27Kip1 expression was immunohistochemically analysed in 51 normal tissue samples, 11 nodular hyperplasias (NH), 22 high-grade prostatic intraepithelial neoplasias (PIN), 56 localized prostate adenocarcinomas, and 19 metastases. Immunoblotting was performed in ten cases. Normal prostate epithelium and NH showed diffuse and intense p27Kip1 nuclear expression in most cases. A significant p27Kip1 down-regulation was observed in many carcinomas when compared with benign epithelium. Forty-seven cases (84 per cent) were low p27Kip1 expressors (<50 per cent positive cells) and nine cases (16 per cent) were high p27Kip1 expressors. p27Kip1 down-regulation was also consistently seen in PIN. Fourteen out of 19 metastases (74 per cent) were low p27Kip1 expressors. Six metastatic samples had their corresponding primary tumour analysed and three cases showed decreased expression in the metastasis. It is concluded that p27Kip1 is constitutively expressed in normal and benign prostatic tissue. This expression is clearly down-regulated in neoplastic progression from the preinvasive lesions through invasive carcinoma and metastases and this therefore occurs in early stages of neoplastic transformation. Copyright © 1999 John Wiley & Sons, Ltd.
- Publication
Journal of Pathology, 1999, Vol 187, Issue 5, p563
- ISSN
0022-3417
- Publication type
Article
- DOI
10.1002/(SICI)1096-9896(199904)187:5<563::AID-PATH292>3.0.CO;2-3