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- Title
Effect of the peri-implantitis on the biological function of osteoblasts obtained from the mandibles.
- Authors
Wu Hao; Liu Hong-chen; Lingling, E.; Liu Na; Zhang Bo; Gao Yuxuan; Feng Lin
- Abstract
Objective To study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts. Methods Primary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen I (Col I) mRNA levels were examined by real-time quantitative polymerase chain reaction. OCN protein levels were determined by Western blot. Results After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue (P<0.05). After 7 d of culture, OCN, Runx2, and Col I mRNA expression decreased (P0.05). The OCN protein levels also decreased (.PO.05). Conclusion Peri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.
- Subjects
OSTEOBLASTS; MANDIBLE; PERI-implantitis; INFLAMMATION; OSTEOCALCIN; MESSENGER RNA; CELL proliferation
- Publication
West China Journal of Stomatology, 2014, Vol 32, Issue 2, p130
- ISSN
1000-1182
- Publication type
Article
- DOI
10.7518/hxkq.2014.02.006