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- Title
The thioredoxin reductase-thioredoxin system is involved in the entry of tetanus and botulinum neurotoxins in the cytosol of nerve terminals
- Authors
Pirazzini, Marco; Bordin, Fulvio; Rossetto, Ornella; Shone, Clifford C.; Binz, Thomas; Montecucco, Cesare
- Abstract
Abstract: Tetanus and botulinum neurotoxins cause paralysis by cleaving SNARE proteins within the cytosol of nerve terminals. They are endocytosed inside acidic vesicles and the pH gradient across the membrane drives the translocation of their metalloprotease L domain in the cytosol. This domain is linked to the rest of the molecule by a single interchain disulfide bridge that has to be reduced on the cytosolic side of the membrane to free its enzymatic activity. By using specific inhibitors of the various cytosolic protein disulfides reducing systems, we show here that the NADPH-thioredoxin reductase-thioredoxin redox system is the main responsible for this disulfide reduction. In addition, we indicate auranofin, as a possible basis for the design of novel inhibitors of these neurotoxins.
- Subjects
THIOREDOXIN reductase (NADPH); THIOREDOXIN; TETANUS toxin; BOTULINUM toxin; CYTOSOL; NERVE endings; PARALYSIS; SNARE proteins
- Publication
FEBS Letters, 2013, Vol 587, Issue 2, p150
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.11.007