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- Title
In vitro reconstitution of monogalactosyldiacylglycerol (MGDG) synthase regulation by thioredoxin
- Authors
Yamaryo, Yoshiki; Motohashi, Ken; Takamiya, Ken-ichiro; Hisabori, Toru; Ohta, Hiroyuki
- Abstract
Abstract: Monogalactosyldiacylglycerol (MGDG), a major membrane lipid of chloroplasts, is synthesized by MGDG synthase (MGD) localized in chloroplast envelope membranes. We investigated whether MGD activity is regulated in a redox-dependent manner using recombinant cucumber MGD overexpressed in Escherichia coli. We found that MGD activity is reversibly regulated by reduction and oxidation in vitro and that an intramolecular disulfide bond(s) is involved in MGD activation. Because thioredoxin efficiently reduced disulfide bonds to enhance MGD activity in vitro, MGD is potentially an envelope-bound thioredoxin target protein in higher plants.
- Subjects
ESCHERICHIA coli; CHLOROPLASTS; LIPIDS; OXIDATION
- Publication
FEBS Letters, 2006, Vol 580, Issue 17, p4086
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.06.050