We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cell proliferation detected using [<sup>18</sup>F]FLT PET/CT as an early marker of abdominal aortic aneurysm.
- Authors
Gandhi, Richa; Cawthorne, Christopher; Craggs, Lucinda J. L.; Wright, John D.; Domarkas, Juozas; He, Ping; Koch-Paszkowski, Joanna; Shires, Michael; Scarsbrook, Andrew F.; Archibald, Stephen J.; Tsoumpas, Charalampos; Bailey, Marc A.
- Abstract
<bold>Background: </bold>Abdominal aortic aneurysm (AAA) is a focal aortic dilatation progressing towards rupture. Non-invasive AAA-associated cell proliferation biomarkers are not yet established. We investigated the feasibility of the cell proliferation radiotracer, fluorine-18-fluorothymidine ([18F]FLT) with positron emission tomography/computed tomography (PET/CT) in a progressive pre-clinical AAA model (angiotensin II, AngII infusion).<bold>Methods and Results: </bold>Fourteen-week-old apolipoprotein E-knockout (ApoE-/-) mice received saline or AngII via osmotic mini-pumps for 14 (n = 7 and 5, respectively) or 28 (n = 3 and 4, respectively) days and underwent 90-minute dynamic [18F]FLT PET/CT. Organs were harvested from independent cohorts for gamma counting, ultrasound scanning, and western blotting. [18F]FLT uptake was significantly greater in 14- (n = 5) and 28-day (n = 3) AAA than in saline control aortae (n = 5) (P < 0.001), which reduced between days 14 and 28. Whole-organ gamma counting confirmed greater [18F]FLT uptake in 14-day AAA (n = 9) compared to saline-infused aortae (n = 4) (P < 0.05), correlating positively with aortic volume (r = 0.71, P < 0.01). Fourteen-day AAA tissue showed increased expression of thymidine kinase-1, equilibrative nucleoside transporter (ENT)-1, ENT-2, concentrative nucleoside transporter (CNT)-1, and CNT-3 than 28-day AAA and saline control tissues (n = 3 each) (all P < 0.001).<bold>Conclusions: </bold>[18F]FLT uptake is increased during the active growth phase of the AAA model compared to saline control mice and late-stage AAA.
- Publication
Journal of Nuclear Cardiology, 2021, Vol 28, Issue 5, p1961
- ISSN
1071-3581
- Publication type
journal article
- DOI
10.1007/s12350-019-01946-y