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- Title
Quantifying vocal fold wound-healing biomechanical property changes.
- Authors
Dion, Gregory R.; Guda, Teja; Mukudai, Shigeyuki; Bing, Renjie; Lavoie, Jean‐Francois; Branski, Ryan C.; Lavoie, Jean-Francois
- Abstract
<bold>Objectives: </bold>Development of novel vocal fold (VF) therapeutics is limited by a lack of standardized, meaningful outcomes. We hypothesize that automated microindentation-based VF biomechanical property mapping matched to histology permits quantitative assessment.<bold>Study Design: </bold>Ex vivo.<bold>Methods: </bold>Twelve anesthetized New Zealand white rabbits underwent endoscopic right VF injury. Larynges were harvested/bisected day 7, 30, or 60 (n = 4/group), with four uninjured controls. Biomechanical measurements (normal force, structural stiffness, and displacement at 1.96 mN) were calculated using automated microindentation mapping (0.3 mm depth, 1.2 mm/s, 2 mm spherical indenter) with a grid overlay (>50 locations weighted toward VF edge, separated into 14 zones). Specimens were marked/fixed/sectioned, and slides matched to measurement points.<bold>Results: </bold>In the injury zone, normal force/structural stiffness (mean, standard deviation [SD]/mean, SD) increased from uninjured (2.2 mN, 0.64/7.4 mN/mm, 2.14) and day 7 (2.7 mN, 0.75/9.0 mN/mm, 2.49) to day 30 (4.3 mN, 2.11/14.2 mN/mm, 7.05) and decreased at 60 days (2.7 mN, 0.77/9.1 mN/mm, 2.58). VF displacement decreased from control (0.28 mm, 0.05) and day 7 (0.26 mm, 0.05) to day 30 (0.20 mm, 0.05), increasing at day 60 (0.25 mm, 0.06). A one-way ANOVA was significant; Tukey's post hoc test confirmed day-30 samples differed from other groups (P < 0.05), consistent across adjacent zones. Zones far from injury remained similar across groups (P = 0.143 to 0.551). These measurements matched qualitative histologic variations.<bold>Conclusion: </bold>Quantifiable VF biomechanical properties can be linked to histology. This technological approach is the first to simultaneously correlate functional biomechanics with histology and is ideal for future preclinical studies.<bold>Level Of Evidence: </bold>NA Laryngoscope, 130:454-459, 2020.
- Subjects
NEW Zealand; VOCAL cords; LARYNGOPLASTY; ONE-way analysis of variance; STANDARD deviations; BIOMECHANICS; LARYNX; VOCAL cord injuries; WOUND healing; BIOLOGICAL models; RABBITS; RESEARCH funding; LARYNGOSCOPY; ANIMALS; KINEMATICS
- Publication
Laryngoscope, 2020, Vol 130, Issue 2, p454
- ISSN
0023-852X
- Publication type
journal article
- DOI
10.1002/lary.27999