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- Title
One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system.
- Authors
Wan, Haifeng; Feng, Chunjing; Teng, Fei; Yang, Shihua; Hu, Baoyang; Niu, Yuyu; Xiang, Andy Peng; Fang, Weizhen; Ji, Weizhi; Li, Wei; Zhao, Xiaoyang; Zhou, Qi
- Abstract
The article focuses on the use of site-specific nucleases such as Transcription Activator-Like Effector Nucleases (TALENs) and Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) for carrying out genome modification in non-human primates (NHPs). It states that p53 tumor suppressor gene, injected via a zygote injection using a CRISPR system, was used to produce biallelic mutant monkeys. It states that addition of desired template DNA to the CRISPR system can modify nucleotide.
- Subjects
PRIMATE genomes; CRISPRS; ANIMAL mutation; PRIMATE genetics; NUCLEASES; NUCLEOTIDES
- Publication
Cell Research, 2015, Vol 25, Issue 2, p258
- ISSN
1001-0602
- Publication type
Article
- DOI
10.1038/cr.2014.158