We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Liquid chromatography coordination ion-spray mass spectrometry (LC–CIS–MS) of docosahexaenoate ester hydroperoxides.
- Authors
Seal, Jennifer R.; Porter, Ned A.
- Abstract
Coordination ion-spray mass spectrometry (CIS–MS) is a useful tool in the detection and identification of complex mixtures of cholesterol ester and phospholipid hydroperoxides. The methyl ester, cholesterol ester, and phospholipid hydroperoxides of docosahexaenoic acid were analyzed by LC–CIS–MS and their elution orders were identified. Their corresponding alcohols were also identified. The methyl hydroperoxydocosahexaenoate (HPDHE) elution order is 14, 17, 16, 13, 20, 11, 10, 4, 7, 8 while the methyl hydroxydocosahexaenoate (HDHE) elution order is 14 ≥ 17, 16, 13, 11, 10, 20, 7, 8, 4. The cholesteryl HPDHE elution order is 14, 17, 16, 13, 20, 11 ≥ 10, 4, 7, 8 and the cholesteryl HDHE elution order is 17, 14, 16, 13, 11, 20, 10, 7, 8, 4. The elution order of the 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphatidylcholine (PDPC) hydroperoxides and alcohols is 20, 16, 17, 13, 14, 10, 11, 7, 8, 4.
- Subjects
PHOSPHOLIPIDS; ORGANIC compounds; PEROXIDES; COMPLEX compounds; LIQUID chromatography; MASS spectrometry
- Publication
Analytical & Bioanalytical Chemistry, 2004, Vol 378, Issue 4, p1007
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-003-2274-6