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- Title
Valency‐Controlled Framework Nucleic Acid Signal Amplifiers.
- Authors
Liu, Qi; Ge, Zhilei; Mao, Xiuhai; Zhou, Guobao; Zuo, Xiaolei; Shen, Juwen; Shi, Jiye; Li, Jiang; Wang, Lihua; Chen, Xiaoqing; Fan, Chunhai
- Abstract
Abstract: Weak ligand–receptor recognition events are often amplified by recruiting multiple regulatory biomolecules to the action site in biological systems. However, signal amplification in in vitro biomimetic systems generally lack the spatiotemporal regulation in vivo. Herein we report a framework nucleic acid (FNA)‐programmed strategy to develop valence‐controlled signal amplifiers with high modularity for ultrasensitive biosensing. We demonstrated that the FNA‐programmed signal amplifiers could recruit nucleic acids, proteins, and inorganic nanoparticles in a stoichiometric manner. The valence‐controlled signal amplifier enhanced the quantification ability of electrochemical biosensors, and enabled ultrasensitive detection of tumor‐relevant circulating free DNA (cfDNA) with sensitivity enhancement of 3–5 orders of magnitude and improved dynamic range.
- Subjects
BIOMOLECULE analysis; NUCLEIC acids; LIGANDS (Chemistry); MOLECULAR recognition; VALENCE (Chemistry)
- Publication
Angewandte Chemie, 2018, Vol 130, Issue 24, p7249
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201802701