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- Title
Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α.
- Authors
Poo, Haryoung; Song, Jae Jun; Hong, Seung-Pyo; Choi, Yoon-Ho; Yun, Sun Woo; Kim, Jin-Ho; Lee, Sang Chul; Lee, Sung-Goo; Sung, Moon Hee
- Abstract
A new E. coli expression system, the pHCE-IIB vector, using a constitutively expressing HCE promoter derived upstream from the d-amino acid aminotransferase (d-AAT) gene of Geobacillus toebii, was developed for the high-level expression of foreign proteins without induction. The expression efficiency of recombinant human tumor necrosis factor-α (rhTNFα) using pHCE-IIB was compared with that of the pET14B vector under the control of a T7 promoter induced by IPTG. The total amount of rhTNFα produced by the pHCE system was approximately twice that produced by the pET system under optimal fermentation conditions thereby demonstrating the convenience and economical advantage of the new constitutive pHCE-IIB expression vector over the conventional pET expression vector.
- Subjects
ESCHERICHIA coli; AMINO acids; CYTOKINES; AMINOTRANSFERASES; TRANSFERASES; TUMOR necrosis factors
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 14, p1185
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1016107230825