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- Title
Cadmium, lead, and zinc removal by expression of the thiosulfate reductase gene from Salmonella typhimurium in Escherichia coli.
- Authors
Bang, San-Weong; Clark, Douglas S.; Keasling, Jay D.
- Abstract
The thiosulfate reductase gene (phsABC) from Salmonella typhimurium was expressed in Escherichia coli in order to produce sulfide from inorganic thiosulfate and precipitate metals as metal sulfide complexes. The sulfide-engineered strain removed significant amounts of heavy metals from the medium within 24 h: 99% of zinc up to 500 μM, 99% of lead up to 200 μM, 99% of 100 μM and 91% of 200 μM cadmium. In a mixture of 100 μM each of cadmium, lead, and zinc, the strain removed 99% of the total metals from solution within 10 h. Cadmium was removed first, lead second, and zinc last. These results have important implications for removal of metals from wastewater contaminated with several metals.
- Subjects
ENTEROBACTERIACEAE; SALMONELLA; ZINC; SALMONELLA typhimurium; ESCHERICHIA coli; THIOSULFATES
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 16, p1331
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005611331948