We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Product quality of a recombinant fusion protein expressed in immobilised baby hamster kidney cells grown in protein-free medium.
- Authors
Cruz, Helder J.; Dias, Elsa M.; Peixoto, Cristina M.; Moreira, Josà L.; Carrondo, Manuel J.T.
- Abstract
Baby hamster kidney cells producing a recombinant IgG-IL2 fusion protein were grown as spinner batch cultures in a protein-free medium, with cells immobilised in porous and non-porous supports at different support concentrations and agitation rates. Product quality, i.e., integrity, decreases up to 60% with the increase in agitation rate or support concentration and from porous to non-porous supports. LDH activity is a good indicator for the monitoring of product degradation, and thus product quality. Optimal conditions regarding titre of intact product were achieved at 60 rpm with 2 g porous support l-1.
- Subjects
CELL culture; PROTEINS; POROUS materials; LACTATE dehydrogenase; PRODUCT quality; IMMUNOGLOBULIN G
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 8, p677
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005631602517