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- Title
Single-Cell Screening through Cell Encapsulation in Photopolymerized Gelatin Methacryloyl.
- Authors
Panneer Selvam, Venkatesh Kumar; Fukunaga, Takeru; Suzuki, Yuya; Okamoto, Shunya; Shibata, Takayuki; Santra, Tuhin Subhra; Nagai, Moeto
- Abstract
This study evaluated the potential of gelatin methacryloyl (GelMA) for single-cell screening compared to polyethylene glycol diacrylate (PEGDA). GelMA photopolymerized at 1000–2000 mJ/cm2 produced consistent patterns and supported HeLa cell viability. GelMA (5%w/v) facilitated better cell collection within 2 days due to its shape retention. GelMA demonstrated biocompatibility with HeLa cells exhibiting exponential proliferation and biodegradation over 5 days. The average cell displacement over 2 days was 16 µm. Two targeted cell recovery strategies using trypsin were developed: one for adherent cells encapsulated at 800 mJ/cm2, and another for floating cells encapsulated at 800 mJ/cm2, enabling the selective removal of unwanted cells. These findings suggest GelMA as a promising biomaterial for single-cell screening applications, offering advantages over PEGDA in cell encapsulation and targeted recovery.
- Subjects
PHOTOPOLYMERIZATION; GELATIN; POLYETHYLENE glycol; BIOCOMPATIBILITY; HELA cells
- Publication
Micro (2673-8023), 2024, Vol 4, Issue 2, p295
- ISSN
2673-8023
- Publication type
Article
- DOI
10.3390/micro4020018