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- Title
Non-invasive tracking of human haemopoietic CD34(+) stem cells in vivo in immunodeficient mice by using magnetic resonance imaging.
- Authors
Niemeyer M; Oostendorp RA; Kremer M; Hippauf S; Jacobs VR; Baurecht H; Ludwig G; Piontek G; Bekker-Ruz V; Timmer S; Rummeny EJ; Kiechle M; Beer AJ; Niemeyer, Markus; Oostendorp, Robert A J; Kremer, Markus; Hippauf, Sandra; Jacobs, Volker R; Baurecht, Hansjörg; Ludwig, Georg
- Abstract
<bold>Objective: </bold>To assess migration of CD34(+) human stem cells to the bone marrow of athymic mice by using magnetic resonance (MR) imaging and Resovist, a contrast agent containing superparamagnetic iron oxide (SPIO) particles.<bold>Methods: </bold>All animal and human procedures were approved by our institution's ethics committee, and women had given consent to donate umbilical cord blood (UCB). Balb/c-AnN Foxn1(nu)/Crl mice received intravenous injection of 1 x 10(6) (n=3), 5 x 10(6) (n=3) or 1 x 10(7) (n=3) human Resovist-labelled CD34(+) cells; control mice received Resovist (n=3). MR imaging was performed before, 2 and 24 h after transplantation. Signal intensities of liver, muscle and bone marrow were measured and analysed by ANOVA and post hoc Student's t tests. MR imaging data were verified by histological and immunological detection of both human cell surface markers and carboxydextrancoating of the contrast agent.<bold>Results: </bold>CD34(+) cells were efficiently labelled by Resovist without impairment of functionality. Twenty-four hours after administration of labelled cells, MR imaging revealed a significant signal decline in the bone marrow, and histological and immunological analyses confirmed the presence of transplanted human CD34(+) cells.<bold>Conclusion: </bold>Intravenously administered Resovist-labelled CD34(+) cells home to bone marrow of mice. Homing can be tracked in vivo by using clinical 1.5-T MR imaging technology.
- Publication
European Radiology, 2010, Vol 20, Issue 9, p2184
- ISSN
0938-7994
- Publication type
journal article
- DOI
10.1007/s00330-010-1773-z