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- Title
BMP-2 regulates the expression of myosin Va via smad in melan-a melanocyte.
- Authors
Park, Ji Yun; Jo, Chan Song; Ku, ChangHoe; Hwang, Jae Sung
- Abstract
Myosin Va (Myo Va) is one of three protein complexes involved in melanosome transport. In this study, we identified BMP-2 as an up-regulator of Myo Va expression using 2-methyl-naphtho[1,2,3-de]quinolin-8-one (MNQO). Our results showed that MNQO reduced the mRNA and protein expression of Myo Va and BMP-2 in melanocytes. Knockdown of BMP-2 by siRNA also affected Myo Va mRNA and protein expression, confirming that MNQO regulates Myo Va through BMP-2. Furthermore, phosphorylation of Smad1/5/8 by BMP2 treatment confirmed that the BMP-2/Smad signaling pathway regulates Myo Va expression in Melan-a melanocytes. Smad-binding elements were found in the Myo Va promoter and phosphorylated Smad1/5/8 bind directly to the Myo Va promoter to activate Myo Va transcription and BMP-2 enhances this binding. These findings provide insight into a new role for BMP-2 in Melan-a melanocytes and a mechanism of regulation of Myo Va expression that may be beneficial in the treatment of albinism or hyperpigmentation disorders.
- Subjects
GENE expression; MYOSIN; SMAD proteins; PROTEIN expression; CELLULAR signal transduction
- Publication
Archives of Dermatological Research, 2024, Vol 316, Issue 6, p1
- ISSN
0340-3696
- Publication type
Article
- DOI
10.1007/s00403-024-02955-9