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- Title
Enhanced production of fructosyltransferase in Aspergillus oryzae by genome shuffling.
- Authors
Wang, Shenghai; Duan, Mengjie; Liu, Yalan; Fan, Sen; Lin, Xiaoshan; Zhang, Yi
- Abstract
Objective: To breed Aspergillus oryzae strains with high fructosyltransferase (FTase) activity using intraspecific protoplast fusion via genome-shuffling. Results: A candidate library was developed using UV/LiCl of the conidia of A. oryzae SBB201. By screening for enzyme activity and cell biomass, two mutants (UV-11 and UV-76) were chosen for protoplast fusion and subsequent genome shuffling. After three rounds of genome recombination, a fusion mutant RIII-7 was obtained. Its FTase activity was 180 U g, approximately double that of the original strain, and RIII-7 was genetically stable. In fermentation culture, FTase activity of the genome-shuffled strain reached a maximum of 353 U g using substrate-feeding method, and this value was approximately 3.4-times higher than that of the original strain A. oryzae SBB201. Conclusions: Intraspecific protoplast fusion of A. oryzae significantly enhanced FTase activity and generated a potentially useful strain for industrial production.
- Subjects
KOJI; FRUCTOSYLTRANSFERASES; PROTOPLASTS; GENOMES; ENZYMES
- Publication
Biotechnology Letters, 2017, Vol 39, Issue 3, p391
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-016-2254-5