We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
中药马勃市场调查及止血活性.
- Authors
李佳萦; 毕雅琼; 包海鹰; 李 玉
- Abstract
The taxonomic composition of puffball mushrooms on Chinese traditional medicine markets were investigated by taking samples from several major traditional medicine markets in China. Water extracts of Calvatia gigantea, C. lilacina and Bovistella utriformis were compared for their hemostatic activity in vivo. Bleeding time (BT) and coagulation time (CT) were determined by the tail-cutting method and the capillary method, respectively. Prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB) in plasma were determined by the hemagglutination assay. ELISA kit was used to measure the contents of coagulation factors in mouse plasma. The results showed that 47 random samples were collected from different markets, and only 14 samples (accounting for 30%) were identified to be Calvatia gigantea and C. lilacina, which were recognized puffball mushrooms in Chinese pharmacopoeia. The remaining 33 samples were Lycoperdon perlatums, Mycenastrum corium and Bovistella utriformis, and B. utriformis was the species with the greatest number of samples (accounting for 64%). Compared with the blank group, the water extracts of C. gigantea, C. lilacina, and B. utriformis reduced both bleeding time and clotting time significantly. The PT, APTT and TT of C. gigantea were significantly decreased. The APTT and TT of C. clilacia were significantly decreased. Fibrinogen was significantly increased in C. gigantea and B. utriformis treated mice. Coagulation factor Ⅻ was extremely significantly increased in C. gigantea and C. lilacina treated mice. C. gigantea water extract also resulted in a significant increase in coagulation factor Ⅹ, coagulation factor Ⅶ, PAI-1, and an extremely significant decrease in t-PA. C. lilacina water extract also resulted in a significant increase in coagulation factor Ⅷ and t-PA. B. utriformis also resulted in a significnat increase in coagulation factor Ⅶ. This study provided a reference for standardizing the puffball mushroom markets in China and utilization of puffball mushroom resources.
- Subjects
CHINA; BLOOD coagulation factors; CHINESE medicine; BLOOD coagulation factor VIII; PARTIAL thromboplastin time; THROMBIN time; FIBRINOGEN
- Publication
Acta Edulis Fungi, 2023, Vol 30, Issue 3, p50
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2023.03.006