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- Title
Plants Possess a Cyclic Mitochondrial Metabolic Pathway similar to the Mammalian Metabolic Repair Mechanism Involving Malate Dehydrogenase and L-2-Hydroxyglutarate Dehydrogenase.
- Authors
Hüdig, Meike; Maier, Alexander; Scherrers, Isabell; Seidel, Laura; Jansen, Erwin E. W.; Mettler-Altmann, Tabea; Engqvist, Martin K. M.; Maurino, Veronica G.
- Abstract
Enzymatic side reactions can give rise to the formation of wasteful and toxic products that are removed by metabolite repair pathways. In this work, we identify and characterize a mitochondrial metabolic repair mechanism in Arabidopsis thaliana involving malate dehydrogenase (mMDH) and L-2- hydroxyglutarate dehydrogenase (L-2HGDH). We analyze the kinetic properties of both A. thaliana mMDH isoforms, and show that they produce L-2-hydroxyglutarate (L-2HG) from 2-ketoglutarate (2-KG) at low rates in side reactions. We identify A. thaliana L-2HGDH as a mitochondrial FADcontaining oxidase that converts L-2HG back to 2-KG. Using loss-of-function mutants, we show that the electrons produced in the L-2HGDH reaction are transferred to the mitochondrial electron transport chain through the electron transfer protein (ETF). Thus, plants possess the biochemical components of an L-2HG metabolic repair system identical to that found in mammals. While deficiencies in the metabolism of L-2HG result in fatal disorders in mammals, accumulation of L-2HG in plants does not adversely affect their development under a range of tested conditions. However, orthologs of L-2HGDH are found in all examined genomes of viridiplantae, indicating that the repair reaction we identified makes an essential contribution to plant fitness in as yet unidentified conditions in the wild.
- Subjects
PLANT mitochondria; PLANT metabolism; MALATE dehydrogenase; EFFECT of poisons on plants; PLANT metabolites; ARABIDOPSIS thaliana
- Publication
Plant & Cell Physiology, 2015, Vol 56, Issue 9, p1820
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pcv108