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- Title
HPLCを用いた畜水産物を主原料とした加工食品中の 残留抗菌性物質分析法の検討
- Authors
Shotaro Hirata; Atsushi Masayama; Tadashi Nakatani; Hidenobu Hoshi; Satoshi Takator
- Abstract
A rapid and easy method for determination of antibacterial substances in processed foods manufactured from livestock and marine products using high performance liquid chromatograph with photodiode anay detector (HPLC-PDA) has been developed. Residual antibacterial substances were extracted with a mixture of acetonitrile/methanol and citrate-phosphate buffer. The lipids were removed from the extract by acetonitrile-hexane partition and solid-phase column. After removal of the solvent, the extract was resolved in potassium phosphate solution and analyzed with HPLC-PDA. Recovery tests of 8 major antibacterial substances from processed foods were performed, and all substances exhibited acceptable recoveries (70-120%) with low relative standard deviations. The time for sample preparation with 8 samples to test solutions was approximately 6 hours. This method could be useful for primary screening inspection of residual antibacterial substances in processed foods manufactured from livestock and marine products.
- Publication
Japanese Journal of Food Chemistry & Safety, 2021, Vol 28, Issue 3, p138
- ISSN
1341-2094
- Publication type
Article