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- Title
BIOASSAY GUIDED HEPATOPROTECTIVE ACTIVITY OF POLYGONATUM CIRRHIFOLIUM AGAINST ISONIAZID AND RIFAMPICIN INDUCED HEPATOTOXICITY IN RATS.
- Authors
Ghai, Roma; Nagarajan, K.; Grover, Parul; Kumar, Vinay; Goel, Richa; Kaur, Charanpreet; Chauhan, Reenu
- Abstract
The present investigation was performed to examine the hepatoprotective effect of the aqueous ethanolic extract of Polygonatum cirrhifolium in antitubercular drug-induced liver damage. P. cirrhifolium rhizomes were crushed, dissolved in various solvents (in order of polarity), and then tested for phytochemicals. Based on their findings, mass extraction utilizing the ethanol-water mixture (50: 50) was carried out using the Soxhlet method. The doses for animal research were established through acute toxicity tests. The hepatoprotective potential of aqueous ethanolic extract (50:50) of rhizomes was determined in Wistar rats at doses of 200 mg kg-1 and 400 mg kg-1 p.o. per day. Blood samples were examined for the biochemical markers SGOT, SGPT, ALP, total bilirubin, and albumin. Histopathology of the liver was also conducted followed by in vitro anti-oxidant studies. Simultaneously, the extract was subjected to LCMS characterization. P. cirrhifolium extract at both the doses 200 mg kg-1 and 400 mg kg-1 has shown significant hepatoprotective activity against hepatotoxicity induced by INH+ RIF in a dose-dependent manner, as depicted by the significant changes in the values of blood biomarkers and in vitro anti-oxidant studies. Histopathological studies showed that the treatment with 200 mg kg-1 and 400 mg kg-1 of P. cirrhifolium exhibited regeneration of liver architecture and portal system by reducing the haemorrhage and inflammatory infiltrate. LC-MS characterization showed serpentine, 5-hydroxy methylfurfural and cephalotaxine as active constituents. It can be inferred that hydroethanolic extract of P. cirrhifolium protects the liver from anti-TB induced toxicity and this protection could be due to the active phytoconstituents.
- Subjects
ISONIAZID; ACUTE toxicity testing; HEPATOTOXICOLOGY; BIOLOGICAL assay; HEPATIC portal system; BIOMARKERS; RIFAMPIN
- Publication
Indian Drugs, 2023, Vol 60, Issue 7, p80
- ISSN
0019-462X
- Publication type
Article
- DOI
10.53879/id.60.07.13557