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- Title
丹参酚酸B 通过Nrf2/HO-1 对脑缺血/再灌注损伤的保护作用研究.
- Authors
卫永鲲; 郭晓华; 王波平; 王卫东; 靳小力; 李健康; 马慧玲
- Abstract
Objective: To study the protective effects and the mechanismof Salvianolic acid B on the cerebral ischemia/reperfusion injury in rats. Methods: An in vivo model of cerebral ischemia 2 h followed by reperfusion was made by reversibly ligating common carotid artery, The male SD rats were randomly divided into control group, vehicle + I/R group and Salvianolic acid B+I/R groupwith ten in each group. The primary of rat cortical neurons were cultured and give the dose of 0, 10, 25, 50 μmol/L Salvianolic acid B, respectively.Through the stained with solution of 2,3,5-triphenyltetrazolium chloride to calculate the infarct volume of brains, use western blot to analyze the expression of protein of Nrf2 and HO-1 in rats and the expression of cytoplasmic and nuclear protein of Nrf2 and total protein of HO-1 in neurons. According to the oxygen-glucose deprivation(OGD) model, detect the cell death and cellular ROS in different dose of Salvianolic acid B and the cell death and cellular ROS after Nrf2 or HO-1 si RNA transfection. Results: Compared with vehicle group, the infarct volumes was significantly decreased in Salvianolic acid B group, and the expression of protein of Nrf2 and HO-1 was increased in rat brains(P<0.05). In rat cortical neurons, the expression of protein HO-1 and nuclear protein of Nrf2 was increased gradually, the expression of cytoplasmic protein of Nrf2 was reduced gradually(P<0.05). In OGD conditions, compared with control group, the cell death and cellular ROS were decreased in Salvianolic acid B group, knockdown of Nrf2 or HO-1, the cell death and cellular ROS were also significantly decreased in Salvianolic acid B group(P<0.05). Conclusions: Salvianolic acid B has the neuroprotection against I/R injury, and the mechanism may be related to its antioxidant activities through Nrf2 /HO-1 pathways.
- Publication
Progress in Modern Biomedicine, 2016, Vol 16, Issue 35, p6817
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2016.35.005