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- Title
MiR-378a-5p 直接靶向 ZEB1上调鼻咽癌 CNE-1 细胞 E-cadherin 表达.
- Authors
LV Qing; SU Hong; WANG Jiu; HUANG Nu-nu; ZHANG Ya-ou
- Abstract
Objective: MiR-378a-5p was identified for decades and its tumor repressive role was demonstrated in various types of cancer including lung cancer, colorectal cancer and breast cancer etc. The relationship between miR-378 overexpression and cell proliferation has been well established. However, no report focused on the cell adhesion change post miR-378 induction. Methods and Results: In this article, we discovered treated cells with miR-378a-5p greatly represses cell migration and invasion in nasopharyngeal carcinoma CNE-1 cells by using wound healing assay and trans-well assay, respectively. We also revealed that E-cadherin expression was significantly elevated upon miR-378a-5p increasing using immunoblot. Finally, we validated that ZEB1, an important negative regulator of E-cadherin, was a direct target of miR-378a-5p by dual luciferase assay. Conclusion: Our result suggests that miR-378a-5p up-regulates E-cadherin expression by repressing ZEB1 expression which finally inhibits Wnt signaling pathway and downstream genes.Together, our study provided a novel understanding of miR-378a-5p tumor suppressive function.
- Subjects
CADHERINS; TUMOR treatment; CELL adhesion
- Publication
Progress in Modern Biomedicine, 2016, Vol 16, Issue 2, p201
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2016.02.001