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- Title
Cloning of Nucleoprotein Gene (NP) of Rinderpest Virus (RPV) RBOK Vaccine Strain.
- Authors
Tonbak, Sükrü; Özdarendeli, Aykut; Bolat, Yusuf
- Abstract
In the present study cloning of the NP gene in a cloning vector is reported. For this purpose, Vero cells were infected with rinderpest RBOK vaccine strain. Total RNA was obtained from the infected cells and reverse-transcription (RT) was performed to get cDNAs of NP gene. Afterwards, the NP gene was amplified by polymerase chain reaction (PCR) with the primers specific to the NP gene. The NP gene, 1575 bp in length, was cloned into pGEM-3Zf (-) cloning vector. PCR screening and enzyme digestion assays carried out to verify the presence of the NP gene.
- Subjects
RINDERPEST virus; RINDERPEST vaccines; NUCLEOPROTEINS; GENES; CLONING; POLYMERASE chain reaction
- Publication
Turkish Journal of Veterinary & Animal Sciences, 2005, Vol 29, Issue 2, p297
- ISSN
1300-0128
- Publication type
Article