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- Title
Direct Electron Transfer of Cellobiose Dehydrogenase on Positively Charged Polyethyleneimine Gold Nanoparticles.
- Authors
Tavahodi, Mojtaba; Ortiz, Roberto; Schulz, Christopher; Ekhtiari, Ali; Ludwig, Roland; Haghighi, Behzad; Gorton, Lo
- Abstract
Efficient conjugation between biomolecules and electrode materials is one of the main challenges in the field of biosensors. Cellobiose dehydrogenase (CDH) is a monomeric enzyme, which consists of two separate domains: one catalytic dehydrogenase domain (DHCDH) carrying strongly bound flavin adenine dinucleotide (FAD) in the active site and a cytochrome domain (CYTCDH) carrying a b-type heme connected by a flexible linker region. Herein, we report on the development of a lactose biosensor, based on direct electron transfer (DET) from CDH from Phanerochaete sordida (PsCDH) electrostatically attached onto polyethyleneimine-stabilized gold nanoparticles (PEI@AuNPs) used to cover a conventional polycrystalline solid gold disk electrode. PEI@AuNPs were synthesized in aqueous solution using PEI as reducing agent for AuIII and as stabilizer for the nanoparticles. The heterogeneous electron-transfer (ET) rate (ks) for the redox reaction of immobilized PsCDH at the modified electrodes was calculated based on the Laviron theory and was found to be (39.6±2.5) s-. The proposed lactose biosensor exhibits good long term stability as well as high and reproducible sensitivity to lactose with a response time less than 5 s and a linear range from 1 to 100 μM.
- Subjects
CHARGE exchange; CELLOBIOSE; DEHYDROGENASES; POLYETHYLENEIMINE; GOLD nanoparticles; CYTOCHROMES
- Publication
ChemPlusChem, 2017, Vol 82, Issue 4, p546
- ISSN
2192-6506
- Publication type
Article
- DOI
10.1002/cplu.201600453