We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Targeted mutagenesis of Drosophila RNaseZ gene by homologous recombination.
- Authors
Andreenkov, O.; Volkova, E.; Demakov, S.; Xie, X.; Dubrovsky, E.; Zhimulev, I.
- Abstract
For the first time we used a homologous recombination method to obtain complete and precise deletion of Drosophila dRNaseZ gene. In the founder line of flies in which the RNaseZ sequence was replaced by attP site, the full-length sequence of the gene was reintegrated, and its functionality was shown. This approach will allow us to generate further gene mutations in different domains of dRNaseZ protein and discover a broad spectrum and uncover functions outside of tRNA processing.
- Subjects
DROSOPHILA genetics; ENDORIBONUCLEASES; SITE-specific mutagenesis; TRANSFER RNA; GENETIC recombination
- Publication
Doklady Biochemistry & Biophysics, 2016, Vol 471, Issue 1, p399
- ISSN
1607-6729
- Publication type
Article
- DOI
10.1134/S1607672916060065