We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bacterial keratitis: Photodynamic inactivation reduced experimental inflammation.
- Authors
DEICHELBOHRER, MONA; MING-FENG WU; SEITZ, BERTHOLD; HUTTENBERGER, DIRK; LASCHKE, MATTHIAS W.; FOTH, HANS-JOCHEN; WONNENBERG, BODO; WAGENPFEIL, STEFAN; MEIER, CAROLA; BISCHOFF, MARKUS; TSCHERNIG, THOMAS
- Abstract
The successful treatment of bacterial keratitis remains an unsolved clinical problem. The current study aimed to establish a murine keratitis model and to investigate the effect of chlorin e6 (Ce6) and photodynamic inactivation (PDI) on corneal inflammation. The cornea of anesthetized mice was scratched and covered with a bacterial suspension of Pseudomonas aeruginosa. A paste containing Ce6 was applied to the cornea with subsequent exposure to specified light. Two days later the animals were sacrificed, and the globes were processed for light microscopy. Evaluation parameters were the maximal corneal thickness and the severity of the hypopyon. The maximal corneal thickness of 290±16 µm in the infected and untreated group was significantly reduced to 220±8 µm in the infected and treated group (P<0.05). In addition, the hypopyon was less severe in the infected and treated group. In conclusion, the present study indicates that PDI using Ce6 may be a potential approach to treat patients suffering with severe bacterial keratitis.
- Subjects
BACTERIAL keratitis; INFLAMMATION prevention; PHOTODYNAMIC therapy; CORNEA injuries; PSEUDOMONAS aeruginosa; BACTERIAL disease treatment
- Publication
Experimental & Therapeutic Medicine, 2017, Vol 14, Issue 5, p4509
- ISSN
1792-0981
- Publication type
Article
- DOI
10.3892/etm.2017.5109