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- Title
S almonella acquires ferrous iron from haemophagocytic macrophages.
- Authors
Nagy, Toni A.; Moreland, Sarah M.; Detweiler, Corrella S.
- Abstract
Bacteria harbour both ferrous and ferric iron transporters. We now report that infection of macrophages and mice with a S almonella enterica Typhimurium strain containing an inactivated feoB -encoded ferrous iron transporter results in increased bacterial replication, compared to infection with wild type. Inactivation of other cation transporters, SitABCD or MntH, did not increase bacterial replication. The feoB mutant strain does not have an intrinsically faster growth rate. Instead, increased replication correlated with increased expression in macrophages of the fepB -encoded bacterial ferric iron transporter and also required siderophores, which capture ferric iron. Co-infection of mice with wild type and a feoB mutant strain yielded a different outcome: FeoB is clearly required for tissue colonization. In co-infected primary mouse macrophages, FeoB is required for S . Typhimurium replication if the macrophages were IFNγ treated and contain phagocytosed erythrocytes, a model for haemophagocytosis. Haemophagocytes are macrophages that have engulfed erythrocytes and/or leucocytes and can harbour S almonella in mice. These observations suggest that S almonella acquires ferrous iron from haemophagocytic macrophages.
- Subjects
SALMONELLA genetics; GENETIC regulation; IRON content of bacteria; PHAGOCYTOSIS; BACTERIAL replicons; MACROPHAGES; CYANOBACTERIA
- Publication
Molecular Microbiology, 2014, Vol 93, Issue 6, p1314
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.12739