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- Title
Molecular characterization of laccase genes from the basidiomycete Trametes hirsuta Bm-2 and analysis of the 5′ untranslated region (5′UTR).
- Authors
Pereira-Patrón, Alejandrina; Solis-Pereira, Sara; Lizama-Uc, Gabriel; Ramírez-Prado, Jorge H.; Pérez-Brito, Daisy; Tapia-Tussell, Raul
- Abstract
The aim of this study was to identify and characterize laccase genes produced by Trametes hirsuta Bm-2 in a liquid medium, both with and without induction. The amplification of 5′and 3′regions of laccase sequences was obtained by the RACE-PCR method, and these were assembled to obtain a cDNA of total length. Two new laccase genes were isolated from basal medium (lac-B) and lignocellulosic grapefruit substrate (lac-T), both encoding open reading frames of 2566 bp. Both laccase-predicted proteins consisted of 521 amino acids, four copper-binding regions, a signal peptide, and five potential glycosilation sites (Asn-Xaa-Ser/Tre). Moreover, the deduced amino acid sequences share about 76–85% identity with other laccases of WRF. Sequence comparison showed 47 synonymous point mutations between lac-B and lac-T. In addition, 5′ untranslated regions (UTR) of laccase genes lac-B and lac-T showed differences in length and number of regulatory elements that may affect transcriptional or translational expression of these genes.
- Subjects
LACCASE; AMINO acid sequence; GENES; GENE expression
- Publication
3 Biotech, 2019, Vol 9, Issue 4, pN.PAG
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-019-1691-y