We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
CORRELATIVE ANALYSIS OF LIPID CONTENTS AND RELATIVE GENE EXPRESSION BETWEEN CHICKEN BREEDS.
- Authors
Ahmed, Irfan; Zhengtian Li; Xiaohua Duan; Dahai Gu; Tengfei Dou; Ying Huang; Changrong Ge; Xi Zhang; Junjing Jia
- Abstract
The muscle composition of fatty acid has appeared as economically important traits due to their profound effect in meat quality. It has been shown that breed and age are most important factors effect on the muscle fatty acid composition in chickens. However, the molecular mechanism of fatty acid composition between the local Chinese chicken and commercial broiler which showed different growing rate is still unclear. To address the molecular mechanism underlying of muscle fatty acid composition, we comparably analyzed the fatty acid (FA) compositions in breast and thigh muscle with different ages by using an Agilent 6890 Series EGC system gas chromatograph equipped with a HP Innowax (30 m × 0.32 mm inside diameter) crosslinked polyethylene glycol column and FID detector (Agilent Technologies, USA) in Chinese native Wuding chickens and fast-growing Cobb broiler. Furthermore, quantitative reverse transcription PCR (Polymerase Chain Reaction) (RT-qPCR) was performed to investigate expression pattern of related candidate genes of fatty acid composition including D9D (Delta-9- Desaturase), FADS-2 (Fatty Acid Desaturase-2), FASN (Fatty Acid Synthatase), FATP-1 (fatty acid transport protein-1) and LPL (lipoprotein lipase) in relative breast and thigh muscles. The results showed that Wuding chickens showed both higher levels of SFA and USFA in two type muscles and two time points due to higher muscle lipid contents compare with Cobb broiler. Thus, Wuding chickens had approximately twice to three folds amount of MUFA (monounsaturated fatty acid) and PUFA (polyunsaturated fatty acid) and association with higher abundance expression of FADS-2, D9D and FATP-1 mRNA in two type of muscles and at two time points compare to broilers. In contrast, broiler showed low amount of MUFA and PUFA and association with higher abundance expression LPL mRNA in two type muscles and at two time points. Similar with reports in fish, rat and human, the FADS-2 and D9D gene code protein might played important role in operation as Δ6 or Δ9 desaturases to synthesis USFAs, including Oleic acid (C18:1), Linoleic acid (LA, C18:2n-6), a-Linolenic acid (ALA, C18:3 n-6) and Docosahexaenoic acid (C22:6n-3, DHA) in chicken muscles. Breed, age and muscle type had significantly influence on the muscle fatty acids composition and expression profiling of related lipid metabolism or fatty acid synthase gene. It is obviously that FADS-2, D9D, FATP-1, FASN and LPL genes play important role in regulating muscle fatty acid synthase and influence in meat quality in chicken.
- Subjects
DELTA State (Nigeria); POULTRY breeding; MONOUNSATURATED fatty acids; LIPID analysis; LINOLENIC acids; UNSATURATED fatty acids; GENE expression; DOCOSAHEXAENOIC acid; FISH oils
- Publication
Pakistan Journal of Agricultural Sciences, 2019, Vol 56, Issue 4, p1095
- ISSN
0552-9034
- Publication type
Article
- DOI
10.21162/PAKJAS/19.9201