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- Title
Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats.
- Authors
Wang, Kai; Wan, Zhengrui; Wu, Liming; Wang, Bei; Yang, Qinqin; Ma, Quanxin; Chen, Minli
- Abstract
Salvianolic acid A (SAA) is an active phenolic acid derived from <italic>Salvia miltiorrhiza</italic> Bunge (Danshen). To explore whether SAA has a therapeutic effect against inflammatory bowel disease (IBD), an acute colitis model was induced in rats by administering 3% dextran sodium sulphate (DSS) for one week. SAA in doses of 4 and 8 mg/kg/day was given by tail vein injection during DSS administration. Both dosages of SAA ameliorated the colitis symptoms, with decreases observed in the disease activity index. A high dosage of SAA (8 mg/kg/day) promoted a longer colon length and an improved colonic tissue structure, compared with the DSS-treated rats not receiving SAA. SAA dose-dependently decreased colonic gene expression of pro-inflammatory cytokines (<italic>IL-1β</italic>, <italic>MCP-1</italic> and <italic>IL-6</italic>). Moreover, a high dosage of SAA protected against DSS-induced damage to tight junctions (TJ) in the rats’ colons, by increasing TJ-related gene expression (<italic>ZO-1</italic> and <italic>occuldin</italic>). Finally, using 16S rRNA phylogenetic sequencing, we found that SAA modulated gut microbiota imbalance during colitis by increasing the gut microbial diversity as well as selectively promoting some probiotic populations, including <italic>Akkermansia</italic> spp. Our study suggests that SAA is a promising candidate for the treatment of IBD.
- Subjects
ANIMAL experimentation; COLITIS; COLON (Anatomy); CYTOKINES; DEXTRAN; DOSE-effect relationship in pharmacology; GENE expression; INFLAMMATORY mediators; INFLAMMATORY bowel diseases; INTRAVENOUS injections; INTERLEUKINS; PHENOLS; RATS; GUT microbiome; PROBIOTICS; SEQUENCE analysis; PHARMACODYNAMICS
- Publication
Nutrients, 2018, Vol 10, Issue 6, p791
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu10060791