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- Title
Early oleate deficiency leads to severe defects in fetal rat liver development.
- Authors
Mohammadzadeh, Fatemeh; Alihemmati, Alireza; Tazehkand, Abbas Pirpour; Darabi, Masoud; Mehdizadeh, Amir
- Abstract
Objective(s): Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat liver development. Materials and Methods: Female Wistar rats with an average weight of 200±20 g were subjected to this study. After mating, pregnant rats were divided into three groups and gavaged with the vehicle, MF 438 or MF-438 plus oleate from day 3 of pregnancy for five days. Obtained fetuses were sacrificed and the liver tissues were retrieved. Hepatic morphological index, biochemical markers, and gene expression of hepatic development markers were analyzed using Hematoxylin-Eosine, spectrometry, and real-time PCR techniques, respectively. Results: Relatively, deficient morphological indices and hepatic maturation markers were observed in fetus livers of the inhibitor-treated group. In comparison to the other two groups, total hepatic protein and glycogen content were increased with treatment of MF-438 plus oleate. Hepatocyte nuclear factor 1α, alpha fetoprotein, albumin, and cytochrome P450 gene expression were also significantly increased in the group treated with both MF-438 and oleate. Conclusion: Our data indicate that oleate availability during early embryo development is linked with fetal rat liver development.
- Subjects
ELLAGIC acid; HEPATOCYTE nuclear factors; LIVER; ALPHA fetoproteins; BIOMARKERS; CYTOCHROME P-450
- Publication
Iranian Journal of Basic Medical Sciences, 2019, Vol 22, Issue 9, p1010
- ISSN
2008-3866
- Publication type
Article
- DOI
10.22038/ijbms.2019.35084.8345