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- Title
Calcium- and hydroxyapatite-binding properties of glucuronic acid-rich and iduronic acid-rich glycosaminoglycans and proteoglycans.
- Authors
Embery, Graham; Rees, Sarah; Hall, Rachel; Rose, Keith; Waddington, Rachel; Shellis, Peter
- Abstract
This study describes the interaction of a small chondroitin sulphate proteoglycan and the glycosaminoglycans chondroitin 4-sulphate, dermatan sulphate and heparan sulphate with hydroxyapatite. All macromolecules possessed a high affinity, with the iduronic acid-rich dermatan sulphate and heparan sulphate displaying higher adsorption maxima than the glucuronic acid-rich chondroitin 4-sulphate. At similar concentrations, dermatan sulphate produced a 30% inhibition of hydroxyapatite-induced crystal growth, whilst chondroitin 4-sulphate yielded 50% inhibition. Estimation of the calcium binding capacity of these glycosaminoglycans using equilibrium dialysis indicated that chondroitin 4-sulphate bound five times more calcium than dermatan sulphate at a calcium concentration similar to that of serum. The data indicate a possible important role for chondroitin 4-sulphate in dentinogenesis where it is the dominant glycosaminoglycan, since it could act as a capture point for calcium ions during mineralisation, with the leucine-rich domain of its parent proteoglycan acting as anchor points to type I collagen.
- Subjects
HYDROXYAPATITE; GLUCURONIC acid; GLYCOSAMINOGLYCANS; PROTEOGLYCANS; CALCIUM; CHONDROITIN sulfates; DERMATAN sulfate; CRYSTAL growth
- Publication
European Journal of Oral Sciences, 1998, Vol 106, p267
- ISSN
0909-8836
- Publication type
Article
- DOI
10.1111/j.1600-0722.1998.tb02186.x