We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Streptococcus mutans / Sigara dumanı kondensatının Streptococcus mutans tarafından üretilen eksopolisakkaritlerin üretimi ve karakterizasyonu üzerine etkisi
- Authors
Xu, Chun ping; Zeng, Ying; Shentu, Hongqian; Zhao, Aijing; Mao, Duobin
- Abstract
Objective: Streptococcus mutans is regarded as the major agent causing dental caries. It has been well documented that cigarette smoke affects the growth of S. mutans. This study investigate the effect of cigarette smoke condensate (CSC) on the production and characterization of exopolysaccharides (EPS) produced by S. mutans ATCC 35668. Methods: Cigarettes of Shanhua brand were used to prepare the CSC. S. mutans was cultured in MSB media with the addition of CSC under anaerobic condition. Furthermore, the EPS fraction was isolated and purified by gel filtration chro-matography on Sepharose CL-6B. The molecular characterization of EPS was analyzed by GC-MS, FT-IR and size exclusion chromatography/multiangle laser light scattering (SEC/MALLS) system. Results: The results showed that CSC at tested concentrations could significantly increase the growth of S. mutans and acetic acid production, compared with the control. The CSC was not found to affect carbohydrate composition of the EPS, but the molecular mass of EPS decreased from 3.04×104 g/mol (without CSC) to 2.75×104 (with CSC). The SEC/MALLS also revealed the molecular conformation of EPS changed from flexible coil to globular shape in aqueous solution. Conclusion: This study revealed that CSC was directly able to affect molecular mass and structural conformation of EPS from S. mutans. The molecular characterization of EPS would become an indicator in certain pathological disorders.
- Subjects
PHYSIOLOGICAL effects of tobacco; MICROBIAL exopolysaccharides; STREPTOCOCCUS mutans; GEL permeation chromatography; SEPHAROSE
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2016, Vol 41, Issue 4, p269
- ISSN
0250-4685
- Publication type
Article
- DOI
10.1515/tjb-2016-0039