We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cryopreservation of the coccolithophore, Emiliania huxleyi (Haptophyta, Prymnesiophyceae).
- Authors
Aude Houdan; Benoît Véron; Pascal Claquin; SéBastien Lefebvre; Jean-Marc Poncet
- Abstract
We studied the cryopreservation of the most common coccolithophore, Emiliania huxleyi which is considered as one of the main global carbon cycle participants. Both stages of this complex life cycle species were submitted to gradual addition of three distinct cryoprotectants: dimethylsulfoxide (7.5% v/v), methanol (5% v/v) and proline (0.5 M). They were then control-rate cooled (−5 °C min−1) to −50 °C before plunging into liquid nitrogen. Free radical oxygen species have been proposed to occur in cells subjected to pre-freezing manipulation or to cooling. Therefore, catalase (preventing accumulation of hydroxyl radicals) was evaluated for its ability to improve cell viability before and after freezing-thawing challenge.
- Publication
Journal of Applied Phycology, 2005, Vol 17, Issue 5, p413
- ISSN
0921-8971
- Publication type
Article