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- Title
Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets.
- Authors
J. PospÃÅ¡ilová; H. Synková; D. Haisel; P. BaÅ¥ková
- Abstract
Abstract  The aim of this research was to determine whether exogenous abscisic acid (ABA) applied immediately after ex vitro transfer of in vitro grown plants can improve their acclimatization. Tobacco (Nicotiana tabacum L.) plantlets were transferred into pots with Perlite initially moistened either by water or 50 µM ABA solution and they were grown under low (LI) or high (HI) irradiance of 150 and 700 µmol mâ2 sâ1, respectively. Endogenous content of ABA in tobacco leaves increased considerably after ABA application and even more in plants grown under HI. Stomatal conductance, transpiration rate and net photosynthetic rate decreased considerably 1 d after ex vitro transfer and increased thereafter. The gas exchange parameters were further decreased by ABA application and so wilting of these plants was limited. Chlorophyll (a) and β-carotene contents were higher in ABA-treated plants, but the content of xanthophyll cycle pigments was not increased. However, the degree of xanthophyll cycle pigments deepoxidation was decreased what also suggested less stress in ABA-treated plants. No dramatic changes in most chlorophyll a fluorescence parameters after ex vitro transfer suggested that the plants did not suffer from restriction of electron transport or photosystem damage.
- Subjects
PLANT-water relationships; SOIL moisture; PLANT water requirements; EFFECT of water levels on plants; WATER requirements of tree farms
- Publication
Biologia Plantarum, 2009, Vol 53, Issue 1, p11
- ISSN
0006-3134
- Publication type
Article