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- Title
Nimodipine rescues N-methyl-N-nitrosourea-induced retinal degeneration in rats.
- Authors
Dan Wang; Yan Li; Zhuo Wang; Gui-yuan Sun; Qiu-hua Zhang
- Abstract
Background: That nimodipine (NMD) is potentially useful for ophthalmic treatment. However, the effect of NMD is unknown on retinal degenerative diseases. Objective: The purpose of the present study was to investigate the effect of NMD on N-methyl-N-nitrosourea (MNU)-induced retinal degeneration (RD) and elucidate its possible mechanisms. Materials and Methods: Morphological observation of NMD on MNU-induced RD was evaluated by light microscopy and electron microscopy. Nonenzymatic antioxidant glutathione (GSH) was measured by a colorimetric method. Transforming growth factor-beta (TGF-β) was measured by enzyme-linked immunosorbent assay (ELISA). Telomerase was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results: The significantly protective effect of NMD on MNU-induced RD was demonstrated morphologically. NMD increased the content of GSH and decreased the level of TGF-β in rat retina. RT-PCR analysis demonstrated that NMD treatment significantly decreased mRNA level of telomerase. Conclusion: These data suggest that NMD inhibit MNU-induced RD in rats. The expressions of TGF-β, telomerase and GSH contents might partially contribute to its protective effects on MNU-induced RD.
- Subjects
NIMODIPINE; N-methylnicotinamide; NITROSOUREAS; RETINAL degeneration treatment; OPHTHALMIC drugs; TRANSFORMING growth factors-beta
- Publication
Pharmacognosy Magazine, 2013, Vol 9, Issue 34, p149
- ISSN
0973-1296
- Publication type
Article
- DOI
10.4103/0973-1296.111276